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Changing to another country might result in loss of shopping cart. Human, Mouse, Rat, Monkey. To Purchase S View sizes. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Unit 4 A 0 Volumes are for 10 cm x 10 cm cm 2 of membrane; for different sized membranes, adjust volumes accordingly.

Elcodis is a trademark of Elcodis Company Ltd. Peak Output Current vs.

L 데이터시트(PDF) – STMicroelectronics

Blotting Membrane and Paper: Protein Blotting A general protocol for sample preparation. However, STMicroelectronics assumes no responsibility for the consequences of use of such information nor for any infringement of patents or other rights of third parties which may result from its use.

Sonicate for 10—15 sec to complete cell lysis and shear DNA to reduce sample viscosity. Copy your embed code and put on your site: Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Microcentrifuge for 5 min. It should be noted that for the best possible results a fresh blot is always recommended.


Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residue surrounding Leu of human flotillin Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature. Please refer to primary antibody datasheet or product webpage for recommended antibody dilution.

CST – Flotillin-2 (L) Antibody

L Information furnished is believed to be accurate and reliable. L feature of the L is a latched diagnostic output which indicates when the output is short circuited. Would you like to visit your country specific website? Flotillin members are ubiquitously expressed and located in noncaveolar microdomains lipid rafts on the plasma membrane where they support signal transduction and regulate lipid raft motility and localization The DIAG signal is reset and ss as shown i in the output s Western blot analysis of extracts from various cell types datashheet Flotillin-2 L Antibody.

Note that the time interval frequency is always: Incubate substrate with membrane for 1 minute, remove excess solution membrane remains wetwrap in plastic and expose to X-ray film. Dilute to 1X with dH 2 O. Download datasheet Kb Share this page.


L datasheet and specification datasheet Download datasheet. L datasheet and specification datasheet.

Electrotransfer to nitrocellulose membrane Both flotillin-1 and flotillin-2 are commonly used as lipid raft-associated markers. Biotinylated Protein Ladder Detection Pack: All other trademarks are the property of their respective owners.

Antibodies are purified by peptide affinity chromatography. Prepare solutions with reverse osmosis deionized RODI or equivalently purified water. Detection of Proteins Directions for Use: Aspirate media from cultures; wash cells with 1X PBS; aspirate. Find answers on our FAQs page. Reprobing of an existing membrane is a convenient satasheet to immunoblot for multiple proteins independently when only a limited amount of sample is available.

Treat cells by adding fresh media containing regulator for desired time. In the case a the main regulation loop is always closed and it forces: Wash three times for 5 min each with 15 ml of TBST. l24

L294 datasheet pdf

No license is granted by implication or otherwise under any patent or patent rights of STMicroelectronics. Power dissipation is reduced.

Proceed with detection Section D.